frame selector addgene plasmid (Addgene inc)
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frame selector addgene plasmid
Frame Selector Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frame+selector+addgene+plasmid/pSPgRNA+(Plasmid+%2347108)/pm40010349-207-187-188
Average 96 stars, based on 164 article reviews
Frame Selector Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frame+selector+addgene+plasmid/pSPgRNA+(Plasmid+%2347108)/pm40010349-207-187-188
Average 96 stars, based on 164 article reviews
frame selector addgene plasmid - by Bioz Stars,
2026-09
96/100 stars
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Cell Recovery:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This SYBR Green Assay:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This Reverse Transcription:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This DNA Extraction:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This EdU Assay:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This Real-time Polymerase Chain Reaction:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This Recombinant:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This Plasmid Preparation:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This Software:Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MYH-1485 MedChemExpress Cat# HY-B0795 Doxycycline Sigma-Aldrich Cat# D1822 Norepinephrine (NE) Sigma-Aldrich Cat# A9512 Cholera toxin (CTX) Sigma-Aldrich Cat# C8052 5-Bromo-2’-deoxyuridine (BrdU) Sigma-Aldrich Cat# B5002 TrypLE (TrypLETM Express Enzyme) Thermo Fisher Cat# 12605010 Opti-MEMTM Thermo Fisher Cat# 31985070 Corning Cell Recovery Solution Sigma-Aldrich Cat# CLS354253 4% Formaldehyde solution Sigma-Aldrich Cat# 1.00496 DAPI (4’,6-Diamidine-2’-phenylindole dihydrochloride) Sigma-Aldrich Cat# 10236276001 Critical Commercial Assays iQTM SYBR Green Supermix BioRad Cat# 1708887 GoScriptTM reverse transcriptase kit Promega Cat# A5000 Miniprep DNA isolation kit Thermo Fisher Cat# K210003 Midiprep DNA isolation kit Thermo Fisher Cat# K210005 EdU Assay kit Thermo Fisher Cat# C10337 NucleoSpin RNA kit Macherey-Nagel Cat# 740955.50 Deposited Data Single-cell RNA-sequencing dataset (Human intestinal organoid cells in ‘‘differentiation medium’’ +/- IFN-g) This paper GEO: GSE242765 Experimental Models: Organoid lines Donor# P11N van de Wetering67 Diakonessen Hospital Utrecht Donor# 12339 He et al.13 The Netherlands Cancer Institute (NKI) Donor# 3466N He et al.13 NKI Donor# 4403N This paper NKI Oligonucleotides Sequences of qPCR primers, see Table S1 This paper N/A Sequences of sgRNAs, see Figures S1, S6, and S7 This |